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chk1 inhibitor chir 124  (Selleck Chemicals)
95
Selleck Chemicals chk1 inhibitor chir 124
TMM in pediatric GBM cell lines and their sensitivity to ATR and <t>CHK1</t> inhibitor. ( A ) Dot blot of the C-Circle assay to test for ALT activity in different pediatric cell lines. Saos2 cells were used as a positive control and HeLa cells were used as negative control for ALT. ( B ) TRAP assay showing telomerase activity in pediatric GBM cell lines. HeLa cells were used as positive control. ( C ) Evaluation of hTERT expression by qPCR. Error bars represent the standard error of the mean from two different experiments run in triplicates. ( D ) Western blot analysis of CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1, ɣH2AX, and total H3 in pediatric GBM cell lines, HeLa, Saos2, and HFF cells. β-Actin served as a loading control. Irradiated HFF cells with 5 Gy were used as a positive control for CHK1 activation cells. ( E ) Proliferation assay for ATR kinase inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of ATR inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines ( F ) proliferation assay for CHK1 inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of <t>CHK1</t> <t>kinase</t> inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines. ( G ) Western blot analysis of ATR T1989-P, ATR, CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1 in our pediatric GBM cell lines treated either with ATR or <t>CHK1</t> <t>kinase</t> inhibitor at predetermined IC 50 for 48 h. β-Actin served as a loading control.
Chk1 Inhibitor Chir 124, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chk1 inhibitor chir 124/product/Selleck Chemicals
Average 95 stars, based on 1 article reviews
chk1 inhibitor chir 124 - by Bioz Stars, 2026-02
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chk1-specific inhibitor chir-124  (Axon Medchem LLC)
90
Axon Medchem LLC chk1-specific inhibitor chir-124
TMM in pediatric GBM cell lines and their sensitivity to ATR and <t>CHK1</t> inhibitor. ( A ) Dot blot of the C-Circle assay to test for ALT activity in different pediatric cell lines. Saos2 cells were used as a positive control and HeLa cells were used as negative control for ALT. ( B ) TRAP assay showing telomerase activity in pediatric GBM cell lines. HeLa cells were used as positive control. ( C ) Evaluation of hTERT expression by qPCR. Error bars represent the standard error of the mean from two different experiments run in triplicates. ( D ) Western blot analysis of CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1, ɣH2AX, and total H3 in pediatric GBM cell lines, HeLa, Saos2, and HFF cells. β-Actin served as a loading control. Irradiated HFF cells with 5 Gy were used as a positive control for CHK1 activation cells. ( E ) Proliferation assay for ATR kinase inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of ATR inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines ( F ) proliferation assay for CHK1 inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of <t>CHK1</t> <t>kinase</t> inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines. ( G ) Western blot analysis of ATR T1989-P, ATR, CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1 in our pediatric GBM cell lines treated either with ATR or <t>CHK1</t> <t>kinase</t> inhibitor at predetermined IC 50 for 48 h. β-Actin served as a loading control.
Chk1 Specific Inhibitor Chir 124, supplied by Axon Medchem LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chk1-specific inhibitor chir-124/product/Axon Medchem LLC
Average 90 stars, based on 1 article reviews
chk1-specific inhibitor chir-124 - by Bioz Stars, 2026-02
90/100 stars
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chk1 inhibitor chir 124  (Axon Medchem LLC)
90
Axon Medchem LLC chk1 inhibitor chir 124
TMM in pediatric GBM cell lines and their sensitivity to ATR and <t>CHK1</t> inhibitor. ( A ) Dot blot of the C-Circle assay to test for ALT activity in different pediatric cell lines. Saos2 cells were used as a positive control and HeLa cells were used as negative control for ALT. ( B ) TRAP assay showing telomerase activity in pediatric GBM cell lines. HeLa cells were used as positive control. ( C ) Evaluation of hTERT expression by qPCR. Error bars represent the standard error of the mean from two different experiments run in triplicates. ( D ) Western blot analysis of CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1, ɣH2AX, and total H3 in pediatric GBM cell lines, HeLa, Saos2, and HFF cells. β-Actin served as a loading control. Irradiated HFF cells with 5 Gy were used as a positive control for CHK1 activation cells. ( E ) Proliferation assay for ATR kinase inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of ATR inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines ( F ) proliferation assay for CHK1 inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of <t>CHK1</t> <t>kinase</t> inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines. ( G ) Western blot analysis of ATR T1989-P, ATR, CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1 in our pediatric GBM cell lines treated either with ATR or <t>CHK1</t> <t>kinase</t> inhibitor at predetermined IC 50 for 48 h. β-Actin served as a loading control.
Chk1 Inhibitor Chir 124, supplied by Axon Medchem LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chk1 inhibitor chir 124/product/Axon Medchem LLC
Average 90 stars, based on 1 article reviews
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chk1 2 inhibitors azd7762  (Selleck Chemicals)
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Selleck Chemicals chk1 2 inhibitors azd7762
FIGURE 1. Treatment with pan-Chk inhibitor <t>AZD7762</t> impairs nuclear localization of 724
Chk1 2 Inhibitors Azd7762, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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azd7762 chk1 chk2 inhibitor  (Selleck Chemicals)
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Selleck Chemicals azd7762 chk1 chk2 inhibitor
FIGURE 1. Treatment with pan-Chk inhibitor <t>AZD7762</t> impairs nuclear localization of 724
Azd7762 Chk1 Chk2 Inhibitor, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chir 124 chk1 inhibitors  (Selleck Chemicals)
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Selleck Chemicals chir 124 chk1 inhibitors
FIGURE 1. Treatment with pan-Chk inhibitor <t>AZD7762</t> impairs nuclear localization of 724
Chir 124 Chk1 Inhibitors, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chk1 inhibitors sch900776  (Selleck Chemicals)
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Selleck Chemicals chk1 inhibitors sch900776
FIGURE 1. Treatment with pan-Chk inhibitor <t>AZD7762</t> impairs nuclear localization of 724
Chk1 Inhibitors Sch900776, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chk1 inhibitors sch900776 - by Bioz Stars, 2026-02
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TMM in pediatric GBM cell lines and their sensitivity to ATR and CHK1 inhibitor. ( A ) Dot blot of the C-Circle assay to test for ALT activity in different pediatric cell lines. Saos2 cells were used as a positive control and HeLa cells were used as negative control for ALT. ( B ) TRAP assay showing telomerase activity in pediatric GBM cell lines. HeLa cells were used as positive control. ( C ) Evaluation of hTERT expression by qPCR. Error bars represent the standard error of the mean from two different experiments run in triplicates. ( D ) Western blot analysis of CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1, ɣH2AX, and total H3 in pediatric GBM cell lines, HeLa, Saos2, and HFF cells. β-Actin served as a loading control. Irradiated HFF cells with 5 Gy were used as a positive control for CHK1 activation cells. ( E ) Proliferation assay for ATR kinase inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of ATR inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines ( F ) proliferation assay for CHK1 inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of CHK1 kinase inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines. ( G ) Western blot analysis of ATR T1989-P, ATR, CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1 in our pediatric GBM cell lines treated either with ATR or CHK1 kinase inhibitor at predetermined IC 50 for 48 h. β-Actin served as a loading control.

Journal: Cancers

Article Title: Alternative Lengthening of Telomeres in Pediatric High-Grade Glioma and Therapeutic Implications

doi: 10.3390/cancers15123070

Figure Lengend Snippet: TMM in pediatric GBM cell lines and their sensitivity to ATR and CHK1 inhibitor. ( A ) Dot blot of the C-Circle assay to test for ALT activity in different pediatric cell lines. Saos2 cells were used as a positive control and HeLa cells were used as negative control for ALT. ( B ) TRAP assay showing telomerase activity in pediatric GBM cell lines. HeLa cells were used as positive control. ( C ) Evaluation of hTERT expression by qPCR. Error bars represent the standard error of the mean from two different experiments run in triplicates. ( D ) Western blot analysis of CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1, ɣH2AX, and total H3 in pediatric GBM cell lines, HeLa, Saos2, and HFF cells. β-Actin served as a loading control. Irradiated HFF cells with 5 Gy were used as a positive control for CHK1 activation cells. ( E ) Proliferation assay for ATR kinase inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of ATR inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines ( F ) proliferation assay for CHK1 inhibitor-treated pediatric GBM cells. Cells were treated with the indicated concentrations of CHK1 kinase inhibitor for 6 days. Proliferation was determined by the WST1 assay. Error bars represent SD; experiment was performed in duplicate. ALT−, Telo+ are ALT negative and telomerase positive cell lines, while ALT+, Telo− are ALT positive and telomerase negative cell lines. ( G ) Western blot analysis of ATR T1989-P, ATR, CHK1-S345-P (ATR phosphorylation site), CHK1-S296-P (autophosphorylation site), total CHK1 in our pediatric GBM cell lines treated either with ATR or CHK1 kinase inhibitor at predetermined IC 50 for 48 h. β-Actin served as a loading control.

Article Snippet: ATR inhibitor VE-822 (S7102) and CHK1 inhibitor (Chir-124) (S2683) were purchased from Selleckchem.

Techniques: Dot Blot, Activity Assay, Positive Control, Negative Control, TRAP Assay, Expressing, Western Blot, Phospho-proteomics, Control, Irradiation, Activation Assay, Proliferation Assay

FIGURE 1. Treatment with pan-Chk inhibitor AZD7762 impairs nuclear localization of 724

Journal: Journal of Virology

Article Title: Activation of the Chicken Anemia Virus Apoptin Protein by Chk1/2 Phosphorylation Is Required for Apoptotic Activity and Efficient Viral Replication

doi: 10.1128/jvi.00936-16

Figure Lengend Snippet: FIGURE 1. Treatment with pan-Chk inhibitor AZD7762 impairs nuclear localization of 724

Article Snippet: The CHK1/2 inhibitors AZD7762 and CHIR-124 were 143 purchased from Selleck (Houston, TX) and used at the indicated concentrations.

Techniques: